[3dem] Calcium free filter paper

[Jing Wang]

Thank you all for the suggestion!! I'll try soaking the filter paper with 1
mM EGTA and dry it out flat. Let you know later if it works,
Cheers,
Jing


On Fri, Aug 29, 2014 at 8:49 PM, <rkhayat at ccny.cuny.edu> wrote:

> Hi,
>
> How about adding EDTA to your complex to chelate the calcium,
> or wash the filter paper with EDTA and let it dry in a vacuum
> before use.
>
> Best wishes,
> Reza
>
> Reza Khayat, PhD
> Assistant Professor
> The City College of New York
> Department of Chemistry, MR-1135
> 160 Convent Avenue
> New York, NY  10031
> Tel. (212) 650-6070
> www.khayatlab.org
>
>
> ---- Original message ----
> >Date: Fri, 29 Aug 2014 18:22:06 -0400
> >From: 3dem-bounces at ncmir.ucsd.edu (on behalf of Jing Wang
> <jing at bu.edu>)
> >Subject: [3dem] Calcium free filter paper
> >To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
> >
> >   Hi there,
> >   I'm working with a complex of calcium sensor
> >   protein, which dissembles upon exposure to calcium.
> >   The complex fell apart after freezing and I
> >   suspected it might due to the** residual calcium
> >   from the vitrobot blotting paper. Is there a
> >   commercially available calcium-free filter paper or
> >   is there a protocol for cleaning up the regular
> >   filter paper? Any comment will be greatly
> >   appreciated!!
> >   Jing
> >________________
> >_______________________________________________
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> >3dem at ncmir.ucsd.edu
> >https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
>
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