[3dem] Calcium free filter paper

[rkhayat at ccny.cuny.edu]

Hi,

How about adding EDTA to your complex to chelate the calcium, 
or wash the filter paper with EDTA and let it dry in a vacuum 
before use.

Best wishes,
Reza

Reza Khayat, PhD
Assistant Professor
The City College of New York
Department of Chemistry, MR-1135
160 Convent Avenue
New York, NY  10031
Tel. (212) 650-6070
www.khayatlab.org


---- Original message ----
>Date: Fri, 29 Aug 2014 18:22:06 -0400
>From: 3dem-bounces at ncmir.ucsd.edu (on behalf of Jing Wang 
<jing at bu.edu>)
>Subject: [3dem] Calcium free filter paper  
>To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
>
>   Hi there,
>   I'm working with a complex of calcium sensor
>   protein, which dissembles upon exposure to calcium.
>   The complex fell apart after freezing and I
>   suspected it might due to the** residual calcium
>   from the vitrobot blotting paper. Is there a
>   commercially available calcium-free filter paper or
>   is there a protocol for cleaning up the regular
>   filter paper? Any comment will be greatly
>   appreciated!!
>   Jing
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