[3dem] Seeking Feedback on the Requirements for Users to Fulfiled to be trained to do Cryo-TEM

David Belnap dbelnap at cores.utah.edu
Wed Aug 13 09:19:55 PDT 2014

I have implemented a similar idea, including the 30 hours of "practice" with dry samples and practical tests (http://www.cores.utah.edu/?page_id=3934).  I think the 30 hours of usage gives users a good amount of time on the microscope to be comfortable enough with its basic operations.  Then, the user can concentrate on the added issues of handling a frozen specimen.  As for your concern about excessive charges, cryo-TEM is a delicate and challenging endeavor. Potential users need to make a serious commitment to learn it well.  Having them first spend time learning basics demonstrates that commitment.

David Belnap

David M. Belnap
Electron Microscopy Core Laboratory
Department of Biochemistry
Department of Biology
2501 SMBB
University of Utah
Salt Lake City, Utah 84112 USA
Phone  801.585.1242
FAX  801.587.3077
David.Belnap at utah.edu<mailto:David.Belnap at utah.edu>

On Aug 12, 2014, at 11:18 AM, YY YY wrote:

Dear All: A very good day to you! I would like to gather some feedback to understand what are the requirements implemented for users who are interested to do cryo-TEM. For example, are they suppose to do some staining on their samples to do some pre-screening process before they decide that their sample are suitable for cryo-tem? Or as long as they have clock a certain number of TEM usage experience, they can be trained to do-cryo TEM?

At the moment, my lab is deciding to implement at least a 30 hours of usage on TEM to gather a certain level of experience such as basic alignment e.g objective astigmatism correction, as well as image taking. Thereafter, they need to do a practical test. If they passed the test, they can be trained to do cryo-tem. However, i am not sure if this would be reasonable such as if 30 hours will be too long as usage will incur some charges as well as time.

All feedbacks are welcome!

Best Regards,
Yee Yan, Tay
Nanyang Technological University

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