[3dem] RE: adsorption problems?

Eric Gilles Hanssen ehanssen at unimelb.edu.au
Sun Oct 13 23:22:16 PDT 2013

HI Michael,
You have probably done it but just in case: have changed the charge of your grids? Eg. Glow discharge in the presence of methanol?

Dr Eric Hanssen

Senior Research Fellow - Manager Advanced Microscopy Facility - Bio21 node
Adjunct Senior Fellow - Department of Biochemistry and Molecular Biology

Bio21 Molecular Science and Biotechnology Institute
30 Flemington Road - The University of Melbourne - Victoria 3010 - Australia
email: ehanssen at unimelb.edu.au<mailto:ehanssen at unimelb.edu.au> | Office: +61 3 83442449 | Microscope: +61 3 83442509 | Fax: +61 3 9347 8265
Web: www.microscopy.unimelb.edu.au<http://www.microscopy.unimelb.edu.au/>

From: 3dem-bounces at ncmir.ucsd.edu [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of Michael Landsberg
Sent: Monday, 14 October 2013 4:46 PM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] adsorption problems?

Hi list members,

We have two complexes that we can separate by gradient centrifugation (+/- a single subunit).  One of them we see clearly on negatively stained grids but the other, which appears more abundant by all other measures, consistently yields grids with no particles.

I am keen to hear from list members with regards to how you typically troubleshoot apparent problems with adsorption, or alternative thoughts as to what might be causing this problem?

Thanks in advance,

Dr Michael J Landsberg
Senior Research Officer
NHMRC Chief Investigator

Institute for Molecular Bioscience
Division of Chemistry and Structural Biology
The University of Queensland
St Lucia, Qld, 4072, AUSTRALIA

Phone: +61 7 3346 2010
E-mail: m.landsberg at imb.uq.edu.au<mailto:m.landsberg at uq.edu.au>
Skype: michael.landsberg

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