[3dem] "leopard skin" ice

[Jing Wang]

Hi Frank,
>From what I heard, It might be caused by uneven freezing, the contraction
or expansion of metal is faster than carbon, so that after freezing, you
end up with larger carbon film rippling on the grid. Make sure the ethane
is cool enough...
Jing


On Wed, Nov 20, 2013 at 1:54 AM, <frankpolzer at physik.hu-berlin.de> wrote:

> Dear all,
>
> During the last weeks, I frequently observe what I believe is so-called
> leopard skin ice in my vitrified samples (plunge frozen in ethane using
> Mark IV Vitrobot).
>
> What again is the reason for this type of ice and how can this be avoided?
>
> Thanks for your help in advance,
>
> Frank
>
>
> Dr. Frank Polzer
>
> TEM Group
> Insitute of Physics
> Humboldt Universität zu Berlin
> Newtonstraße 15
> 12489 - Berlin
> Tel.: +49 30 2093-4995 (office)
> Tel.: +49 30 2093-7829 (TEM)
> Fax: +49 30 2093-7886
>
>
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