[3dem] "leopard skin" ice

[frankpolzer at physik.hu-berlin.de]

Dear all,

During the last weeks, I frequently observe what I believe is so-called
leopard skin ice in my vitrified samples (plunge frozen in ethane using
Mark IV Vitrobot).

What again is the reason for this type of ice and how can this be avoided?

Thanks for your help in advance,

Frank


Dr. Frank Polzer

TEM Group
Insitute of Physics
Humboldt Universität zu Berlin
Newtonstraße 15
12489 - Berlin
Tel.: +49 30 2093-4995 (office)
Tel.: +49 30 2093-7829 (TEM)
Fax: +49 30 2093-7886