[3dem] Re: different mrc formats in relion-1.2 and xmipp-3.???
acheng at scripps.edu
Mon Jul 15 12:58:42 PDT 2013
I want to modify what Roberto stated about distinguishing
single image, stack of images, single volume, stack of volumes in MRC format.
A couple of us made an effort to resolve some mrc conflict with ccp4 library recently.
To look into the future, we designated the ISPG 401 NZ / MZ = N object so not to alter
the original ccp4 definition of MZ ( number of grid (sampling) in a unit cell ). None of us is
aware of the use of MRC format as volume stack, yet. Hopefully when this becomes a
practice, we will all use the same convention.
In short, using the wording of IMGIC 4D
pixel size in Z = CELL(Z) / MZ
single image: ISPG=0 NZ = MZ = 1
image stack: ISPG=0 NZ = N object
single volume: ISPG=1 NZ = MZ = Z slices
volume stack: ISPG=401 MZ = Z slices; NZ / MZ = N object
If any of you have been writing stack of mrc volume and/or have concern over this,
please let me know. I will assemble them to present to the group, or add you to
the discussion if you prefer.
I've presented as a side bar of my poster at 3D-EM GRC the essence of our conclusions.
The full conclusion of the effort will be given in full later after we figure out of a
few of details on handling extended header used in some variant.
Others in the discussion group:
Bridget Carragher (TSRI-Leginon)
Martyn Winn (STFC-CCP4)
Richard Henderson (MRC)
David Matronarde (UColorado-IMOD)
Remco Schoenmakers (FEI)
David Agard (UCSF)
Steven Ludtke (Baylor-EMAN)
-------------- next part --------------
An HTML attachment was scrubbed...
More information about the 3dem