[3dem] freezing tube using Leica HPM100

[Chen Chen]

The air bubbles are a real problem. I tried to use a wire as a syringe or mount the tube to a P10 pipette but still saw holes in the ice. Any thoughts about how to avoid those bubbles?

Thanks,
Chen 
On Feb 14, 2013, at 1:20 PM, John Crum wrote:

> No.  The ends can remain open.  Just make sure the tube is filled with liquid.  Any air will prevent adequate freezing.
> 
> jc
> 
> 
> 
> On 2/13/13 9:16 PM, Chen Chen wrote:
>> Dear Lists,
>> 
>> Has anyone tried to high pressure freeze samples in a tube using Leica HPM100? I am wondering if I need to seal one or both ends of the tube before freezing. Thanks in advance.
>> 
>> Cheers,
>> Chen_______________________________________________
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> 
> -- 
> John Crum
> Automated Molecular Imaging
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