[3dem] freezing tube using Leica HPM100

[Chen Chen]

May I know how you seal the end? Normally I just clamp the tube to make the end flat. Do you think that's enough?

Thanks,
Chen 
On Feb 14, 2013, at 1:18 PM, Cedric Bouchet-Marquis wrote:

> Yes, you have to seal the side that faces the machine when mouting it in its "platic" holder.
> 
> Best
> 
> Cedric
> 
> Sent from my iPhone
> 
> On Feb 13, 2013, at 9:16 PM, "Chen Chen" <c.chen0306 at gmail.com> wrote:
> 
>> Dear Lists,
>> 
>> Has anyone tried to high pressure freeze samples in a tube using Leica HPM100? I am wondering if I need to seal one or both ends of the tube before freezing. Thanks in advance.
>> 
>> Cheers,
>> Chen_______________________________________________
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