[3dem] protein localization

Joachim Frank jf2192 at columbia.edu
Fri Feb 8 08:36:58 PST 2013


Fred Sigworth at Yale developed a way to locate proteins in the membrane 
of vesicles, and to obtain 3D reconstructions using cryo-EM.  Pls. look 
at literature under his name.

--Joachim.

On 2/8/2013 11:32 AM, Reinhard Rachel wrote:
>>>> 08.02.2013 um 16:56:
>> My colleague is working with protein-liposome complexes. She thinks the
>> proteins are either incorporated in the liposomal membrane, or attached to
>> the surface of the liposomes. Is there any EM method, or some other
>> technique, that can be used to assess where the proteins are located? Someone
>> suggested immunogold-labelling. Any thought about this?
> - direct labeling in solution with prim. + second.AB or ProteinA (use small gold, 6nm) and cryo-TEM;
> - freeze-fracture labeling
> both may work fine, in fact.
> kind regards,
> Reinhard
>

-- 
Joachim Frank, Ph.D.
Howard Hughes Medical Institute Investigator,
Professor, Department of Biochemistry and Molecular Biophysics,
and Department of Biology, Columbia University,
650 W. 168th Street, P&S Black Building 2-221
New York, NY10032
(T) 212 305-9510
(F) 212 305-9500
(E) jf2192 at columbia.edu
http://franklab.cpmc.columbia.edu
http://www.hhmi.org/research/investigators/frank.html



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