[3dem] protein localization

[Reinhard Rachel]

>>> 08.02.2013 um 16:56:
> My colleague is working with protein-liposome complexes. She thinks the 
> proteins are either incorporated in the liposomal membrane, or attached to 
> the surface of the liposomes. Is there any EM method, or some other 
> technique, that can be used to assess where the proteins are located? Someone 
> suggested immunogold-labelling. Any thought about this?

- direct labeling in solution with prim. + second.AB or ProteinA (use small gold, 6nm) and cryo-TEM;
- freeze-fracture labeling
both may work fine, in fact. 
kind regards,
Reinhard

-- 
Prof. Dr. Reinhard Rachel
University of Regensburg
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