[3dem] IHRSR in SPARX

[Penczek, Pawel A]

For those who use or plan to use IHRSR (real-space helical reconstruction) implementation in SPARX (sxihrsr) an important note.

We introduced sxhelixboxer (a modified version of e2boxer) that is closely integrated with sxihrsr.

In particular, it has built-in CTF estimation and it also stores in segments's headers information
required for validation of IHRSR results.

The documentation can be found here:
http://sparx-em.org/sparxwiki/sxhelixboxer

and an example how to carry out helical reconstruction is here:
http://sparx-em.org/sparxwiki/Runthrough_helical_example

Description of the methodology:
Behrmann, E., Tao, G., Stokes, D.L., Egelman, E.H., Raunser, S., and Penczek, P.A., 2012. Real-space processing of helical filaments in SPARX. J Struct Biol 177, 302-313.

Regards,
—
Pawel A. Penczek, Ph.D.
Professor
Structural Biology Imaging Center, Director
The University of Texas
phone: 713-500-5416
fax: 713-500-0652
http://www.uth.tmc.edu/bmb/faculty/pawel-penczek.html