[3dem] prepare Formvar Grids

[Denis Chaix]

Hello
We have actualy some troubles to prepare Formvar films.
In fact it's impossible to release the film from the glass slide.
Here you can find our protocol

Formvar solution. 0,4% in chloroform .
Pour the solution into a clean Coplin jar
Dip pre-cleaned slides ( rub thoroughly with Kimwipes) into beaker.
Drain corner on side of beaker.
Blot edge of slide on filter paper.
Score the edges of the Formvar film with an acetone-cleaned razor blade.
Breathe over the length of the slide to loosen the film.
Float the film off onto a clean water surface by slowly immersing the  
slide into the water at a 45° angle.


Have you some tricks or tips to improve this protocol and help the  
release of the film ?
For example, have you some ideas like special brand of slides, or a  
way to clean the slides.

Thanks



Denis
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Chaix Denis <denis.chaix at ibs.fr>
Microscopie Electronique et Méthodes
<http://www.ibs.fr/groupes/groupe-microscopie-electronique-et/>
Room 6234
Institut de Biologie Structurale UMR-5075 <http://www.ibs.fr/spip.php>
41 Rue J. Horowitz
38027 Grenoble Cedex 1,France
Tel: +33.4.38.78.01.59.
Fax: +33.4.38.78.54.94.
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