[3dem] Postdoctoral position open

Ariel Blocker Ariel.Blocker at bristol.ac.uk
Tue Oct 9 02:22:08 PDT 2012

We seek an enthusiastic Postdoctoral Research Assistant/Associate to join
the teams of Ariel Blocker (University of Bristol, United Kingdom) and
Keiichi Namba (University of Osaka, Japan) on a project funded by the UK
Medical Research Council. These laboratories study structural and
mechanistic aspects of thefunction of type III secretion systems in the Gram
negative pathogenic bacterium Shigella flexneri using cryoelectron
microscopy. The project aims to generate an integrated 3D reconstruction of
the isolated T3SS Œneedle complex¹ base, its needle and its distal tip to at
least 10 Å resolution. In parallel, tomographic studies of T3SS within
intact bacteria will also be pursued. Knowledge gained from the project will
contribute to development of new drugs and vaccines against T3SS carrying
The post is funded for 22 to 24 months and will be held primarily within the
School of Cellular and Molecular Medicine in Bristol but including about 25%
time at the Graduate School for Frontier Biosciences in Osaka, with regular
visits to either institution. Experience in electron and cryoelectron
microscopy image acquisition is essential. Substantial, demonstrably
productive, experience in the use of EMAN, SPIDER, Xmipp, IMOD and other
freely available packages for classification and 3D reconstruction purposes
is also necessary. Skills in biochemical techniques for sample preparation
will be added assets.
For further information on the research groups please visit:
<http://www.bris.ac.uk/cellmolmed/staff/blocker.html>  and
<http://www.fbs.osaka-u.ac.jp/eng/labo/09a.html>  . For details of the
Bristol electron microscopy facilities, please visit:
<http://www.bristol.ac.uk/biochemistry/wbif> . The Namba laboratory possess
two helium cooled JEOL 300 kV TEMs with field emission guns and energy
filters, a Titan Krios, all necessary ancillary grid preparation and image
acquisition equipment and PC clusters.
Informal enquiries are welcome and should be made to: Dr. A. Blocker
(ariel.blocker at bristol.ac.uk)

Ariel J. Blocker, Ph.D.
Reader in Microbiology
Schools of Cellular & Molecular Medicine and Biochemistry
Medical Sciences Building
University of Bristol
University Walk
Bristol  BS8 1TD
United Kingdom

Email: ariel.blocker at bristol.ac.uk
Tel office (D39a): +44 117 33 12063
Tel wetlab (D57/D59): +44 117 33 12404
Tel wetlab office (D53): +44 117 33 12059
Tel drylab (D51): +44 117 33 12097
Tel Tecnai20: +44 117 33 12357
Dept. fax: +44 117 3312091
Room: D39a
Personal webpage: 
Group page: http://www.bristol.ac.uk/cellmolmed/infect-immune/blocker/

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