[3dem] CCD retracted when emptying buffer tank

[Jamonshi]

Hi Wim,

Thank you for clarifying. To elaborate my problem here, I notice the camera won't get retracted by buffer cycle while it is taking images, such as live view. But it will be retracted later once It is stopped. We also have Gatan Orius camera installed on the same image plane with Ultrascan, but it is controlled separately by a standalone computer.

Anyway, I got contacted by FEI-TSG regarding to this issue. Hopefully there will be a solution soon.

Regards,
Jian SHI
Manager, cryoEM facility
Center for BioImaging Science
National University of Singapore

On Jun 18, 2012, at 3:05 PM, Wim Hagen <wim.hagen at embl.de> wrote:

> Hi Jian,
> 
> Titan 1.2 and Tecnai 4.x share the same codebase, so I would expect to also see this on Titan. Our Krios runs 1.2.2, the Polara runs 4.4 (beta), the F30 runs 4.2 but it has an Eagle which is non-retractable. I checked things here: on the Krios and Polara, the retractable cameras are not retracted on buffer cycle. Both SerialEM and FEI tomography automatically insert the CCD when the camera is retracted and an acquisition is started.
> 
> I guess the quickest option is to ask Shawn to add the insert command to the acquisition in UCSF tomo.
> 
> Best regards,
> 
> Wim
> EMBL Heidelberg
> 
> On Jun 14, 2012, at 6:53 PM, Jamonshi wrote:
> 
>> Hi Wim,
>> 
>> As I can recall, Tecnai 3.x does not retract CCD when buffer cycle runs. Can you be more specific what version you have? And what type of acquisition software you are referring to? 
>> 
>> To answer Rich, this is our T12, with Ultrascan4000 and Orius, software is Tecnai 4.2 and DM 1.8.3. I am using UCSF tomo to collect data.
>> 
>> Regards,
>> Jian SHI
>> Manager, cryoEM facility
>> Center for BioImaging Science
>> National University of Singapore
>> 
>> On Jun 15, 2012, at 12:24 AM, Wim Hagen <wim.hagen at embl.de> wrote:
>> 
>>> Hi Jian,
>>> 
>>> On all my FEI instruments, the software for the retractable Gatan cameras automatically inserts the camera when it is retracted and an acquisition is taken, so I don't have any problems if a camera get's retracted and not inserted, I actually don't even know if the CCD retraction is happening as my acquisition simply runs, including buffer cycles.
>>> 
>>> Best regards,
>>> 
>>> Wim
>>> 
>>> On Jun 14, 2012, at 6:05 PM, j s wrote:
>>> 
>>>> Dear Lister,
>>>>  
>>>> Our FEI T12 received a software upgrade to Tecnai 4.2. Coming with it there is a unexpected 'feature' (according to FEI): The CCD camera is retracted when the emptying buffer tank start to run, but NOT reinserted afterwards. Such behaviour totally defeats any intention of running unsupervised automatic data collection, including FEI's own software. I am wondering if anyone else had similar experience and how to get around of it. Your help is appreciated.
>>>>  
>>>> Regards,
>>>>  
>>>> Jian SHI
>>>> NUS-CBIS
>>>> _______________________________________________
>>>> 3dem mailing list
>>>> 3dem at ncmir.ucsd.edu
>>>> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
>>> 
>>> -------------------------------------------------------------------
>>>   Wim Hagen
>>>   Senior Engineer Electron Microscopy
>>>   European Molecular Biology Laboratory (EMBL)
>>>   Heidelberg, Germany                    
>>> --------------------------------------------------------------------
>>> 
> 
> 
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