[3dem] Postdoc position at the Institute of Cancer Research, London

[David Barford]

POST DOCTORAL FELLOW IN SINGLE MOLECULE ELECTRON MICROSCOPY

The Institute of Cancer Research
Division of Structural Biology, London

We seek to appoint a post doctoral training fellow to the Division of Structural Biology to undertake single particle electron microscopy analysis of the anaphase promoting complex (APC/C).

Funded by Cancer Research UK the post-doctoral training fellow will join a team investigating the structure and mechanism of the anaphase promoting complex (APC/C), a large macro-molecular multi-subunit assembly that regulates progression through the cell cycle. The aim of this project is to apply single particle cryo-EM techniques to determine high-resolution structures of various functional states of the APC/C (for example apo and ternary complexes of APC/C with co-activator and substrates). Our aim is to achieve resolution sufficient for visualisation of the polypeptide chain and eventually side-chain features. This project extends our existing 10 Å resolution structure and takes advantage of our recombinant over-expression system for reconstituting APC/C (da Fonseca et al., (2011) and Schreiber et al., (2011)). Based within Prof. D. Barford’s group the project will be focussed on EM grid preparation, data acquisition and processing, and 3-dimensional reconstructions with contributions towards sample preparation. The project would suit a highly motivated and committed scientist interested in molecular mechanisms of the cell cycle, cancer biology, structural biology and electron microscopy.

The Division is equipped for cryo-electron microscopy (FEI TF20 and T12) and for protein expression and purification.

The successful applicant will hold at least a PhD (or equivalent) in a biological/physical science. Experience with single particle electron microscopy data acquisition and processing is essential and experience with protein preparations would be an advantage. The post is available from 1st June 2012 (or earlier) for three years in the first instance (subject to programme renewal decision in April). Salary range: £30,289 to £37,254 (the upper scale applies to experienced electron microscopists).

Informal enquires may be made to David Barford (David.Barford at icr.ac.uk).
To apply please see: http://www.icr.ac.uk/jobs/index.shtml  Job ref: 1229219. Closing date: 24th Feb, 2012.
               
References:

Barford, D. (2011). Structural insights into anaphase-promoting complex function and mechanism. Phil Trans Royal Soc B. 366, 3605-3624.

da Fonseca, P. C., Kong, E. H., Zhang, Z., Schreiber, A., Williams, M. A., Morris, E. P. & Barford, D. (2011). Structures of APC/C(Cdh1) with substrates identify Cdh1 and Apc10 as the D-box co-receptor. Nature 470, 274-8.

Schreiber, A., Stengel, F., Zhang, Z., Enchev, R. I., Kong, E. H., Morris, E. P., Robinson, C. V., da Fonseca, P. C. & Barford, D. (2011). Structural basis for the subunit assembly of the anaphase-promoting complex. Nature 470, 227-32.


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Professor David Barford
Division of Structural Biology
Institute of Cancer Research
Chester Beatty Laboratories
237 Fulham Road
London, SW3 6JB
United Kingdom

Tel. +44 (0)20 7153 5420
FAX +44 (0)20 7153 5457
PA (Sonia Malkani) +44 (0)20 7153 5443

E. mail: david.barford at icr.ac.uk

www.icr.ac.uk/davidbarford

The Institute of Cancer Research: Royal Cancer Hospital, a charitable Company Limited by Guarantee, Registered in England under Company No. 534147 with its Registered Office at 123 Old Brompton Road, London SW7 3RP.

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