[3dem] 64bit MRC conversion

Steven Ludtke sludtke at bcm.edu
Thu Jan 5 09:18:52 PST 2012


Boxer, or any other eman1 or 2 program will read virtually any cryoem format. The issue is that the mrc spec has no support for 64 bit images...

Sent from my iPhone

On Jan 5, 2012, at 10:12 AM, Henning Stahlberg <henning.stahlberg at unibas.ch> wrote:

> Hi,
> 
> MRC formats are described here:
> http://ami.scripps.edu/prtl_data/mrc_specification.htm
> 
> and we mirrored that page on 2dx.org, here:
> http://www.2dx.unibas.ch/documentation/mrc-software/mrc-documentation
> 
> The MRC program mrc2tif can do some conversions. 
> 
> Alternatively, bsoft 
> http://lsbr.niams.nih.gov/bsoft/
> http://lsbr.niams.nih.gov/bsoft/programs/bconvert.html
> 
> or EM2EM can do conversions also.
> http://www.imagescience.de/em2em/
> 
> Henning.
> 
> Henning Stahlberg, PhD
> Prof. for Structural Biology, C-CINA, Biozentrum, University Basel
> Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland
> http://c-cina.org | Tel. +41-61-387 32 62
> 
> 
> 
> On Jan 5, 2012, at 6:02 PM, Steven Ludtke wrote:
> 
>> Hi Kat. There is no such thing as a 64 bit mrc file (in the official specs). Where did it come from ? How do you know it is 64 bit ? 64 bit floating point or integer ?
>> 
>> Sent from my iPhone
>> 
>> On Jan 5, 2012, at 9:28 AM, toropova <toropova at fas.harvard.edu> wrote:
>> 
>>> Dear EM community, 
>>> 
>>> Does anyone know how to convert 64-bit MRC image files to a format that Boxer can read, e.g. 8/16-bit TIFF?
>>> 
>>> Many thanks, 
>>> Kat
>>> 
>>> 
>>> Katerina Toropova
>>> Postdoctoral Researcher 
>>> Dept. of Molecular and Cellular Biology
>>> Harvard University
>>> 305 NWB, 52 Oxford St.
>>> Cambridge, MA 02138
>>> 
>>> 
>>> 
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