SV: SV: [3dem] Vitrobot cleaning

[Kimberley Cheng]

Thank you all for the advices! It has been very helpful.

All the best,
Kimberley

________________________________________
Från: Bowman, Valorie D [vdb at purdue.edu]
Skickat: den 10 februari 2012 22:39
Till: Kimberley Cheng
Ämne: Re: SV: [3dem] Vitrobot cleaning

Dear Kimberly,
You shouldn't have any chance of cross contamination if you clean your
forceps between samples and remember to use fresh filter paper.  Nothing
else should touch your grids.

The sample won't contaminate the freezing cup unless you drop a grid after
plunging and let it thaw there.  If you do, just clean the cup with ethanol
after it warms up.

Cheers,

Valorie Bowman
Senior Research Electron Microscopist,

Dept. of Biology
Purdue Univ.
W. Lafayette, In
    47906


 ph. 765-494-5643
 fax 765-496-1189
vdb at purdue.edu



> From: Kimberley Cheng <Kimberley.Cheng at ki.se>
> Date: Fri, 10 Feb 2012 05:22:44 -0500
> To: Christian Geiss <geiss at biophysik.org>, "3dem at ncmir.ucsd.edu"
> <3dem at ncmir.ucsd.edu>
> Subject: SV: [3dem] Vitrobot cleaning
>
> Dear Christian,
>
> I am mostly worried about any risk of cross-contamination of samples. I.e., if
> I freeze sample A today and sample B tomorrow, will it be a risk that my
> sample B specimens can be contaminated with sample A if I haven't cleaned the
> Vitrobot.
>
> Kind regards,
> Kimberley
>
> ________________________________
> Från: 3dem-bounces at ncmir.ucsd.edu [3dem-bounces at ncmir.ucsd.edu] för Christian
> Geiss [geiss at biophysik.org]
> Skickat: den 10 februari 2012 10:57
> Till: 3dem at ncmir.ucsd.edu
> Ämne: Re: [3dem] Vitrobot cleaning
>
> Hi Kimberley,
>
> what kind of contaminations do you have? oil doplets? (cellulose) fibers? etc.
>
> Kind regards,
>
> C. Geiss
>
>
>
> On 02/10/2012 10:45 AM, Kimberley Cheng wrote:
> Dear all,
>
> Has anybody had problems with contamination between freezing sessions when
> using a Vitrobot? If so, any advice on how to eliminate the risk? I've heard
> about decontaminating surfaces using 70% ethanol. Does anybody have any
> experience with this?
>
> Thanks in advance!
> /Kimberley
>
>
>
>
>
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