[3dem] Quantifoil grids

[m.maletta at nki.nl]

Dear all
What about passing them over a flame?

Best
Max
__________________________________
Massimiliano Maletta
post-doc in
NKI Netherlands Cancer Institute -Antoni van Leeuwenhoek Hospital Plesmanlaan 121 B6 1066CX Amsterdam
group of Peter Peters
www.nki.nl/research/peters/<http://www.nki.nl/research/peters/>   | www.necen.nl<http://www.necen.nl/>
m.maletta at nki.nl<mailto:m.maletta at nki.nl>
Tel : +31 20 512 2022
Fax : +31 20 512 2029

________________________________
From: 3dem-bounces at ncmir.ucsd.edu [3dem-bounces at ncmir.ucsd.edu] On Behalf Of Sarah Shahmoradian [shs2284 at gmail.com]
Sent: Friday, 14 December 2012 9:13 PM
To: Isabelle Rouiller
Cc: 3dem at ncmir.ucsd.edu
Subject: Re: [3dem] Quantifoil grids

Dear Dr. Rouiller,

Have you tried washing the grids with acetone?

1) Put filter paper in the bottom of a petri dish
2) Add desired # of grids on top
3) Pour a generous amount of acetone to completely cover the grids
4) Cover petri dish and leave overnight
5) Remove all acetone and wash grids 2x with de-ionized water (gently agitate dish while washing)
6) Remove grids and put them on a new, dry filter paper in another petri dish
7) Leave under fume hood to dry

*You could also wash with ethyl alcohol (gently agitate the dish for 10sec) prior to washing 2x with dH2O.

Hope this helps,

Best regards,
Sarah

---------------------------------------------------------------
Sarah Shahmoradian
Dept. Molecular Physiology & Biophysics
Lab of Wah Chiu
http://ncmi.bcm.edu/
Baylor College of Medicine, Houston TX 77030



On Fri, Dec 14, 2012 at 1:38 PM, Isabelle Rouiller <isabelle.rouiller at mcgill.ca<mailto:isabelle.rouiller at mcgill.ca>> wrote:
Does anyone have a working protocol to remove the plastic from inside the holes of Quantifoil grids?

Washing with chloroform does not work with the ones we presently have.  We also tried flash freezing (it sometimes cracks the plastic, but not always). The distributor (SPI) has been unwilling to exchange them.

Thank you,

Isabelle


Isabelle Rouiller, PhD
Cell Biology Graduate Program Director
Department of Anatomy and Cell Biology
Room 115, Strathcona Anatomy and Dentistry Building
3640 University Street Montreal, Quebec H3A 2B2
(514) 398-3244<tel:%28514%29%20398-3244>


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