[3dem] Image file format of Eagle 4K CCD and conversion

Sacha De Carlo sachadecarlo at yahoo.com
Thu Aug 23 08:24:11 PDT 2012


You can export as TIFF (Full Resolution) from TIA directly, or you can import the *.EMI file in Digital Micrograph and use the Integer 2 byte unsigned data type and set an offset of 126 in the binary offset (assuming a 4096 x 4096 size and depth of 1).

I believe that the free EM2EM image converter from IMAGIC allows you to convert directly as well (I will ask Marin if it works).

Best regards,
Sacha

 
=============================
Dr. Sacha De Carlo
Sr. Res. Scientist
FEI Company


________________________________
 From: "hohu78 at gmail.com" <hohu78 at gmail.com>
To: 3dem at ncmir.ucsd.edu 
Sent: Thursday, August 23, 2012 4:28 PM
Subject: [3dem] Image file format of Eagle 4K CCD and conversion 
 

Dear all,
 
I have installed the Eagle 4K CCD camera from FEI on Tecnai G2 
spirit.
Because I only have experienced Gatan camera, I’m struggling to figure out 
this camera and software.
 
Questions are
1. How to convert emi file format which is the basic option in eagle camera 
to MRC or IMAGIC format?
    Is there any available free software?
2. Using export option in software, I can also save image as several 
formats, 
    like Tif (full resolution), Bmp(full resolution), JPG 
(full resolution), Raw binary (.bin), True image (.ebn). 
    Which file format is the best one for single particle 
analysis without losing the information of image?
 
Anyone who have experiences, give me valuable suggestions, please.
Many thanks,
 
Best regards,
Homin Kim
 
Homin Kim, 
Ph D.
Korea Advanced Institute of Science and Technology (KAIST)
Graduate 
School of Medical Science and Engineering 
BioMedical Research Center(E7) 
Room 2102, KAIST
291 Daehak-ro, Yuseong-gu 
Daejeon, 305-701, Republic of 
Korea
Phone: +82-10-7184-5521(Cellphone), +82-42-350-4244 (Office)
FAX : 
+82-42-350-4240
Email: hm_kim at kaist.ac.kr
_______________________________________________
3dem mailing list
3dem at ncmir.ucsd.edu
https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
-------------- next part --------------
An HTML attachment was scrubbed...
URL: https://mail.ncmir.ucsd.edu/mailman/private/3dem/attachments/20120823/dc86e739/attachment.html


More information about the 3dem mailing list