[3dem] Re: DMSO and vitrified ice

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Hi,every one, 
Does anyone know isolated cell organelle negative-stain for TEM? Need it be
fixed with glutaraldehyde or osmium at first? 

Thanks, 
Lianwan 



rkhayat д: 

> Hi, 
>  
> Does anyone know the maximum amount of DMSO that can be present in a   
> sample when making vitrified ice grids for cryo-EM?  This would be  for  
> single particle analysis.  Thanks. 
>  
> Regards, 
> Reza 
>  
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