[3dem] RE: PCS vs cryoEM

[Philip Köck]

I would question the accuracy of PCS (without knowing how it works) simply based on the
wavelength of visible light.

Philip

From: 3dem-bounces at ncmir.ucsd.edu [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of Kimberley Cheng
Sent: 31 October 2011 10:47
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] PCS vs cryoEM

Dear all,

I recently got the following question "The particle size of our liposome product determined by cryoEM is about 75 nm, but the particle size determined by photon correlation spectroscopy (PCS) is about 110 nm. So we were wondering why the size determined by cryoEM is smaller than by PCS method?".

Does anyone have a good explanation to this?

Many thanks in advance!
/Kimberley
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