[3dem] Positive staining with UA

Terje Dokland dokland at uab.edu
Thu Mar 3 11:08:31 PST 2011

Positive staining is very common with uranyl acetate and is also sample specific. Try PTA or ammonium molybdate, which work more like a pure negative stain.  Also, make sure to glow discharge the grids before use, as  this has a major effect on stain behavior. 

On Mar 3, 2011, at 11:54 AM, shashi bhushan wrote:

> Dear All, 
> We are having problems with the negative staining of our complexes with Uranyl Acetate. We are repeatedly having positive staining instead of negative. We have tried many things without any success. Could some one please provide us some useful tips/detailed protocols to avoid the positive staining. 
> Thanks, 
> Regards, 
> Shashi Bhushan
> <ATT00001..txt>

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