[3dem] Positive staining with UA

[Cedric Bouchet-Marquis]

Did you try defocusing in the microscope?

 

Seriously, if you would give a little more details perhaps people will be
able to help you.

Like what complex you are working with, its size, the percentage of stain
used, the different "things" that you already tried .

 

Cheers 

 

cedric

 

From: 3dem-bounces at ncmir.ucsd.edu [mailto:3dem-bounces at ncmir.ucsd.edu] On
Behalf Of shashi bhushan
Sent: Thursday, March 03, 2011 10:54 AM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] Positive staining with UA

 

Dear All, 

We are having problems with the negative staining of our complexes with
Uranyl Acetate. We are repeatedly having positive staining instead of
negative. We have tried many things without any success. Could some one
please provide us some useful tips/detailed protocols to avoid the positive
staining. 

Thanks, 
Regards, 

Shashi Bhushan

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