[3dem] CCD maintenance

[Jingchuan Sun]

Hi,
Our 4K CCD Gatan camera has ring shaped shadow after install half year. It
could be removed after doing Gain Reference, but appeal again several days
later. It looks like oil and continue changes. Gatan manual suggest to warm
the CCD to remove the oil, otherwise would permanently stain the camera by
electron beam. How often should the CCD be warmed up?

Also should the CCD camera switched off when not in use?

Another question: should the specimen holder with the last cryo grid be left
in goniometer and do the smart heat? Or remove the holder is better?
Thanks!
Jim
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