[3dem] creating models from PDB files
Steven Ludtke
sludtke at bcm.edu
Wed Jul 27 21:13:15 PDT 2011
I would argue that 'better than another' is a relative term. The question is, better for what ?
Are you after 2 A ? 5 A ? 25 A ? Do you need to worry about solvation ? If trying to compare to
a single particle reconstruction, what about angle orientation uncertainty ? That has a very different
effect than the normal sort of blurring most programs do. So, what is it you're after in your
synthetic map ?
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Steven Ludtke, Ph.D.
Associate Professor, Dept. of Biochemistry and Mol. Biol. Those who do
Co-Director National Center For Macromolecular Imaging ARE
Baylor College of Medicine The converse
sludtke at bcm.edu -or- stevel at alumni.caltech.edu also applies
http://ncmi.bcm.edu/~stevel
On Jul 27, 2011, at 2:47 PM, David Gene Morgan wrote:
> Hi,
>
> Has anyone done any sort of critical evaluation of the different
> programs that will read PDB files and generate volumes? I know that
> there are lots of ways to do this, and that different programs appear to
> do fairly different things. But does anyone have good reasons for
> believing that one program is better than another?
>
> --
> David Gene Morgan
> cryoEM Facility
> 320C Simon Hall
> Indiana University Bloomington
> 812 856 1457 (office)
> 812 856 3221 (EM lab)
> http://bio.indiana.edu/~cryo
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