[3dem] Sunday short course on 3D EM at M&M meeting 2011

[Michael Radermacher]

Dear All,

I would like to make you aware of the Sunday (Aug. 7, 8:30 AM-5 PM)  
short course on 3D electron microscopy held at the Microcopy and  
Microanalysis meeting Aug 7-11 in Nashville TN. This course should not  
only be interesting to biologists, but also for people in the material  
sciences field interested in 3D electron microscopy. Registration for  
this course is still open  
(http://www.microscopy.org/MandM/2011/register.cfm). Description below.

X14: 3D Electron Microscopy of Macromolecular Assemblies
Teresa Ruiz, Michael Radermacher, and Stefan Birmanns

This short course will provide a comprehensive description of the  
methods used for 3D structure determination of macromolecular  
complexes from electron micrographs. First specimen preparation  
techniques for single particles (deep stain, vitreous ice), will be  
presented and the selection of imaging conditions including low-dose  
imaging. This will be followed by a detailed explanation of image  
processing techniques with special emphasis on the random conical  
reconstruction technique. In the last part structure interpretation  
and docking of X-ray structures to 3D EM densities will be demonstrated.

The techniques described have application in both biological and  
materials science.

-- 
Michael Radermacher, Ph.D., Prof.
University of Vermont
Dept. Molecular Physiology and Biophysics
HSRF 120 / 149 Beaumont Ave
Burlington, VT 05405
USA

Tel: + 802 656-4834
Fax: + 802 656-0747