[3DEM]file formation change

C.J. biocjh at gmail.com
Thu Jul 14 07:59:54 PDT 2011


Thank you. If I have any question, I will discuss them here again.

2011/7/14 Willy Wriggers <wriggers at biomachina.org>:
> Hi,
>
> I think you misunderstood the tutorial. ASCII is only one of several options
> supported by map2map, it is not "required". Please try to run map2map, the
> program is quite self-explanatory about the format options (see also the
> online user guide).
>
> I no longer suggest to use intermediate SPIDER files as Reza mentioned. I
> contacted various developers about the MRC conventions two years ago and we
> compared programs and fixed various subtle quirks in the programs. So MRC
> maps should now be consistent among the EM programs such as Situs, Chimera,
> EMAN, em2em, etc.
>
> Finally, you can ask me such usage questions directly (I always welcome
> questions, but by broadcasting to everyone at 3DEM - before things are clear
> - you may confuse other Situs users). So feel free to write me directly.
>
> Thanks,
> Willy
>
>
>
>
>
> On 7/12/2011 11:47 PM, C.J. wrote:
>>
>> I also have the similar problem.
>> I have the pdb file and mrc file, but I don't know how to convert the
>> file formation among pdb, mrc, ascii(ascii-formatted density map). As
>> refer to http://situs.biomachina.org/tutorial_classic.html, the ascii
>> formatted density map is required.
>>
>> 2011/7/9 Reza Khayat<rkhayat at scripps.edu>:
>>>
>>> Hi C.J.,
>>>
>>> I would suggest converting the MRC into a spider file then into a Situs
>>> file.  I've had problems converting MRC into Situs format in the past.
>>>
>>> Reza
>>>
>>> ________________________________________
>>> From: 3dem-bounces at ncmir.ucsd.edu [3dem-bounces at ncmir.ucsd.edu] On Behalf
>>> Of C.J. [biocjh at gmail.com]
>>> Sent: Friday, July 08, 2011 12:03 AM
>>> To: 3dem at ncmir.ucsd.edu
>>> Subject: [3DEM]file formation change
>>>
>>> Dear all,
>>>
>>> I have a pdb file which was producted by protein prediction and a mrc
>>> file which was producted by EMAN。
>>> I want to fit the predicted structure(pdb file) into the volumn(mrc
>>> file) through Situs v2.6(download from http://situs.biomachina.org).
>>> How should I do that?
>>> Any advices would be appreciated.
>>>
>>> --
>>> Regards!
>>> C.J.
>>> _______________________________________________
>>> 3dem mailing list
>>> 3dem at ncmir.ucsd.edu
>>> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
>>>
>>
>>
>
>



-- 
Regards!
C.J.


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