[3DEM]asymmetric unit segmentation

Steven Ludtke sludtke at bcm.edu
Wed Jul 13 05:50:13 PDT 2011


While Bajaj's group at UT Austin has some methods they claim to work fully automatically, segmentation almost always
requires some human effort (and additional knowledge) to perform correctly.  I would suggest the 'Segger' tool developed
by Grig Pintilie, now built into Chimera, which is only semi-automatic, but easy to use. Chimera has a 
variety of other useful tools for dealing with symmetry as well, which are at least somewhat documented in the short
videos Tom Goddard put together : http://www.cgl.ucsf.edu/chimera/videodoc/videodoc.html

----------------------------------------------------------------------------
Steven Ludtke, Ph.D.
Associate Professor
Co-Director National Center For Macromolecular Imaging
Dept of Biochemistry and Mol. Biol.
Baylor College of Medicine
sludtke at bcm.edu
stevel at alumni.caltech.edu




On Jul 13, 2011, at 7:39 AM, C.J. wrote:

> Dear all,
> 
> Any one have a experience on segmentation of asymmetric unit from
> icosahedron virus structure volume?
> I wonder whether there is any programme could process that automatically?
> Any comment would be appreciated!
> 
> -- 
> Regards!
> C.J.
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