[3dem] FIB-SEM

[Mike Marsh]

Hi Abrahan,

I don't know if 3DEM mailing-list want to support FIB-SEM community.  If
they do, that is great!

Otherwise, you may consider the recently announced FIB-SEM discussion group
started on Google Groups.  You can find a link here:
http://fibsem.org/index.php?news&nid=8

(I'd love to personally address your question, but I don't have any
background with staining.)

Cheers,
Mike

On Mon, Jan 24, 2011 at 10:37 AM, Abrahan Hernandez <abrahan.hernandez at ki.se
> wrote:

>  Dear 3dem community,
>
>  I am performing data acquisition with a FIB-SEM (Focus ion beam- Scanning
> electron microscope) of metaphase chromosomes in a whole cell. My samples
> are stained pre-embedding with tannic acid (0.15%, for 1 min), Osmium
> tetra-oxide (2%, for 1 hour) and uranyl acetate (1%, fir 1 hour). We can see
> our structures (metaphases chromosomes) but the contrast is not so good,
> therefore I would like to ask you if you have any recommendation for improve
> the pre-embedding staining.
>
>  Best
>   -----
> Abrahan Hernandez Hernandez, PhD
> Postdoc
> Department of Cell and Molecular Biology (CMB)
> Berzelius Väg 35
> Karolinska Institutet
> S-171 77 Stockholm, Sweden
> Phone: +46 (0)8 5248 7396
> Email: abrahan.hernandez at ki.se
>
>
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>
>
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