[3dem] FIB-SEM

[Emily Knauth]

Hi Abrahan,

How are you samples fixed?

 

Cheers,

 

Emily Knauth

Institute for Molecular Bioscience

Division of Chemistry and Structural Biology

The University of Queensland

306 Carmody Road

St Lucia 4072

Brisbane, QLD

Australia

 

Phone: + 61 7 3346 2325

 

 

From: 3dem-bounces at ncmir.ucsd.edu [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of Abrahan Hernandez
Sent: Tuesday, 25 January 2011 2:38 AM
To: 3dem at ucsd.edu
Subject: [3dem] FIB-SEM

 

Dear 3dem community, 

 

I am performing data acquisition with a FIB-SEM (Focus ion beam- Scanning electron microscope) of metaphase chromosomes in a whole cell. My samples are stained pre-embedding with tannic acid (0.15%, for 1 min), Osmium tetra-oxide (2%, for 1 hour) and uranyl acetate (1%, fir 1 hour). We can see our structures (metaphases chromosomes) but the contrast is not so good, therefore I would like to ask you if you have any recommendation for improve the pre-embedding staining.

 

Best

-----

Abrahan Hernandez Hernandez, PhD

Postdoc

Department of Cell and Molecular Biology (CMB)

Berzelius Väg 35

Karolinska Institutet

S-171 77 Stockholm, Sweden

Phone: +46 (0)8 5248 7396

Email: abrahan.hernandez at ki.se

 

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