[3dem] correlative cryostage

[Vladan Lucic]

Dear Cheri and Joachim,

We have a working (FEI) cryo-LM stage in our department. It works with Zeiss 
Axiovert 200M. We did not have to modify the LM, the stage was designed to 
work with this microscope. As far as I know, the more recent Zeiss models need 
to have the stage slightly adjusted. I'm not sure what is advertised regarding 
multiple objectives, but we can change objectives during the operation, if 
that's what you're asking.

The shape of objectives might be important, it works with somewhat conically-
shaped Zeiss objectives. However, I suspect that some of completely flat-top 
objectives couldn't come close enough for a working distance of 2 mm.

Best,

Vladan

Vladan Lucic, PhD
Department of Molecular Structural Biology
Max Planck Institute for Biochemistry
Am Klopferspitz 18
D-82152 Martinsried, Germany

Phone: +49 89 8578-2647
Fax: +49 89 8578-2641



On Monday 24 January 2011 17:28:20 Cheri Hampton wrote:
> 3DEM community,
>   We would like to know if ANYONE has a working FEI LM-EM correlative
> cryo-stage in their labs. If so, which LM does it work with?  Did the
> stage or the LM have to be modified to make it work?  If it works,
> does it work as advertised (with multiple objectives)?
> Thank you,
> 
> Joachim Frank
> Cheri Hampton