[3dem] Summary of Gatan cryoholders CT3500 and 626 responses

[Roseann Csencsits]

You can order the 626 holder with the 3500 transfer station.  I prefer the retaining ring on the 60 degree holder.  If you are planning high tilts, I would suggest considering their new 914 cryo tomography holder.  In sample loading looks like it has some advantages.

Roseann Csencsits, PhD
Supervisor - Donner TEM Facility
Lawrence Berkeley Lab 01-365
1 Cyclotron Road
Berkeley, CA 94720
510-486-4548








On Sep 20, 2010, at 7:30 AM, Heather Holdaway wrote:

> Thank you everyone for your feedback on the Gatan cryoholders.  Most everyone agreed that the CT3500 requires more maintenance and is harder in loading the grid, and the 626 is easier to use and requires less upkeep.  
> 
> Here is a list of the feedback I received regarding the Gatan cryoholders, CT3500 and 626:
> 
> 1. We have both. As other people may tell you, the CT3500 is supposed to be very stable (as with any holder... provided you get a good one) but is more finicky to handle. Grid transfer is a bit more cumbersome than with the 626 and the valve for the dewar's vacuum can be sensitive; one of my students had several vacuum crashes in the holder because of that. Despite the complications, I actually like the transfer station of the CT3500; it has a feature that drips liquid nitrogen on the grid all the time and that seems like a good thing. The clip ring is admittedly a pain from time to time. 
> 
> -- 
> Heather Holdaway
> Electron Microscope Manager
> Cleveland Center for Membrane and Structural Biology
> 
> Mailing Address:
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> 
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