[3dem] Summary of Gatan cryoholders CT3500 and 626 responses

[Heather Holdaway]

Thank you everyone for your feedback on the Gatan cryoholders.  Most
everyone agreed that the CT3500 requires more maintenance and is harder in
loading the grid, and the 626 is easier to use and requires less upkeep.

Here is a list of the feedback I received regarding the Gatan cryoholders,
CT3500 and 626:

1. We have both. As other people may tell you, the CT3500 is supposed to be
very stable (as with any holder... provided you get a good one) but is more
finicky to handle. Grid transfer is a bit more cumbersome than with the 626
and the valve for the dewar's vacuum can be sensitive; one of my students
had several vacuum crashes in the holder because of that. Despite the
complications, I actually like the transfer station of the CT3500; it has a
feature that drips liquid nitrogen on the grid all the time and that seems
like a good thing. The clip ring is admittedly a pain from time to time.

-- 
Heather Holdaway
Electron Microscope Manager
Cleveland Center for Membrane and Structural Biology

Mailing Address:
Case Western Reserve University
School of Medicine
Department of Pharmacology
Wood Building, Room 151
10900 Euclid Avenue
Cleveland, Ohio 44106-4965

Lab and Office Address:
CCSB/West Quad, Room 111H
1819 E 101st St
Cleveland, Ohio 44106

Phone: 216.368.5752
Cell: 765-586-4192
Fax: 216.368.1300
Email: heather.holdaway at case.edu
http://ccmsb.case.edu/
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