[3dem] Help on particles automatic boxing

Steven Ludtke sludtke at bcm.edu
Sun Oct 3 10:55:12 PDT 2010

I would say it depends very much on what you're picking. If you are picking something like a spherical
virus particle then there are fully automatic tools like Ethan which do a near-perfect job. Other approaches
are semi-automatic. Possibilities include:

boxer in EMAN1
e2boxer in EMAN2

For free cryo-EM software, this should be the definitive reference :


Steven Ludtke, Ph.D.
Associate Professor, Dept. of Biochemistry and Mol. Biol.      Those who do
Co-Director National Center For Macromolecular Imaging	           ARE
Baylor College of Medicine                                     The converse
sludtke at bcm.edu  -or-  stevel at alumni.caltech.edu               also applies

On Oct 3, 2010, at 4:50 AM, jh wrote:

> Dear all,
> Anyone could introduce me with a free software, which can
> automatically box particles from cryo-EM images.
> Thanks.
> C.J.
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