[3dem] TEMs and sample preferences

Wright, Elizabeth R. erwrigh at emory.edu
Thu Nov 18 19:31:10 PST 2010


Dear 3DEM Colleagues,

We are in the final stages of setting up our two new microscopes and I have a little question about samples. The 200 kV microscope will be dedicated to the imaging of cryo-specimens. However, we also have a new 120 kV microscope. What are the current preferences about specimens to image with an instrument? The three basic specimen types we image in our EM core lab are cryo, standard sections, and negatively stained samples. Does anyone have information on the types and amounts of contamination that are associated with each? Along with maintenance costs (perhaps)? We also have another TEM we use for basic training and routine screening.

Any and all input is most appreciated.

Sincerely,

Liz
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