[3dem] energy filters on FEI TF-20 (cryo)?

[Ishikawa Takashi]

Dear Chuck,

Yes. We are using a GIF with our TF20. It improves the image quality and is indispensable for our tomography of flagella/cilia (~300nm thickness). The installation was straightforward. But we bought a TF20 and a GIF at the same time. If you are planning to add a GIF later, it would be better to ask FEI if any rearrangement is needed.

Best regards,
Takashi

Takashi Ishikawa 
Research Scientist / Group Leader 
Department of Biology 
Swiss Federal Institute of Technology (ETH Zürich) 
ETH Hönggerberg, HPK F18
CH-8093 Zürich, Switzerland 
phone: +41 44-633-2462 
Fax:  : +41 44-633-1073 
e-mail: takashi.ishikawa at mol.biol.ethz.ch 
Web:  http://www.mol.biol.ethz.ch/groups/ishikawa_group


-----Original Message-----
From: 3dem-bounces at ncmir.ucsd.edu [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of Charles Sindelar
Sent: Donnerstag, 1. April 2010 15:29
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] energy filters on FEI TF-20 (cryo)? 

Hi all,
I am wondering whether anyone here has installed an energy filter  
(i.e. Gatan GIF) on a 200KV FEI F20.

My suspicion is that it should be straightforward, and significantly  
improve performance with tomography (in the zero-loss mode).

It would nice to have confirmation of this, however.  Does anyone have  
direct experience?  Or is it that 300kV instruments are universally  
used in cryo applications with the GIF?

Thanks for your help!

Chuck Sindelar
Brandeis University
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