[3dem] simulated micrograph for ctf determination?

[Steven Ludtke]

There are several programs which will do this. For my part, you can  
use ctfit or applyctf  in EMAN1. However,
even with realistic pink noise added, these images still remain an  
imperfect simulation of real images. Different
programs use different strategies for the raw image processing  
(working with the whole micrograph, boxed out
particles, boxed out carbon film, etc.), so it's hard to come up with  
a single uniform test for them all...

----------------------------------------------------------------------------
Steven Ludtke, Ph.D.
Associate Professor, Dept. of Biochemistry and Mol. Biol.      Those  
who do
Co-Director National Center For Macromolecular Imaging	           ARE
Baylor College of Medicine                                     The  
converse
sludtke at bcm.edu  -or-  stevel at alumni.caltech.edu               also  
applies
http://ncmi.bcm.edu/~stevel

On Sep 10, 2009, at 4:55 PM, jinghua tang wrote:

> To test various programs for determine the CTF, or simply the  
> defocus, does anyone have
> a way to generate a simulated micrograph in which different CTF was  
> applied?
>
> Thank you in advance for your kind helps!
>
> Jinghua
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> 3dem at ncmir.ucsd.edu
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