[3dem] converting large date set using em2em

michael michael at ImageScience.de
Wed Nov 11 09:40:17 PST 2009

Dear Zongli,

I created a large IMAGIC file containing more than 116000 images of size 
128x128 (> 7 Gigabyte). I used em2em to convert this IMAGIC file into an 
MRC file. The resulting MRC file has a correct size.

Jude Short from MRC/LMB kindly send me a MRC program to read MRC 
headers. The print-out showed that all header values were set correctly: 
image size, the number of images in the file, and other header values. 
So em2em worked without any error.

As far as I understood you checked your MRC file using an EMAN tool. May 
be you can check if this program has a problem with the large image size 
or the large number of images.



Zongli Li wrote:
> Dear all,
> I have been trying to convert a large data set (> 6G) of boxed 
> particle images from IMAGIC stack file to MRC stack file using IMAGIC 
> em2em, but always get an invalid MRC output file. The program works 
> just fine for small data set. Does anyone knows how to fix the problem 
> or have other way to convert the large data set from IMAGIC stack to 
> MRC stack?
> Thanks a lot! Any help or suggestion will be highly appreciated.
> Zongli



  Michael Schatz
  Image Science Software GmbH

  Gillweg 3
  D - 14193 Berlin

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  Tel: +49 30 8909 5326
  Fax: +49 30 8909 5321
  URL: www. ImageScience .de

  Geschäftsführer/Managing Director
  Michael Schatz

  HRB: 33106 Berlin-Charlottenburg
  VAT/USt-Id-Nr. DE 136613484


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