[3dem] Registration and 3-D Reconstruction

[Steven Ludtke]

It's a very active field of research. The question is which  
methodology you are interested in, each has its own specialties:

- single particle reconstruction - sets of very noisy images of  
individual ~identical nanoscale objects -> 3D reconstruction
- 2D crystallography - 3D reconstructions from crystals, like x-ray  
crystallography, but with 2-D rather than 3-D crystals
- helical reconstruction - reconstruction using packed arrays with  
helical symmetry
- tomography - VERY noisy 3D reconstructions of possibly unique  
objects, if the objects are ~identical then 3D registration and  
averaging can proceed

I would suggest starting out with Joachim Frank's introductory book :  
"Three-dimensional Electron Microscopy", then perhaps looking at the  
special issue of
JSB from a couple of years ago. The one containing this:

Tang, G., Peng, L., Baldwin, P.R., Mann, D.S., Jiang, W., Rees, I. &  
Ludtke, S.J., 2007, EMAN2: an extensible image processing suite for  
electron microscopy, Journal of structural biology, 157(1), pp. 38-46.

It has articles on many of the current software packages. My group  
develops one of them (EMAN). There is some raw data available from our  
website
http://ncmi.bcm.edu

----------------------------------------------------------------------------
Steven Ludtke, Ph.D.
Associate Professor
Co-Director National Center For Macromolecular Imaging
Dept of Biochemistry and Mol. Biol.
Baylor College of Medicine
sludtke at bcm.edu
stevel at alumni.caltech.edu
http://ncmi.bcm.edu/~stevel



On Jul 7, 2009, at 4:53 AM, Arthur Goshtasby wrote:

>
> Hello everyone,
>
>
> I am a new member and my interest is in image registration and 3-D  
> reconstruction. My background is actually computer vision but have  
> recently become interested in microscopic image analysis. I have  
> found such image fascinating. I am wondering if anyone else is  
> interested in or currently working on the problem. I have some  
> methodologies worked out for registration and 3-D reconstruction and  
> would like to try them on different data sets. If you are working  
> with microscopic image sets and don't mind sharing a set or two with  
> me, I promise to share the obtained results and the methodology that  
> is employed to obtain them with you? I can be reached via phone  
> (937-775-5170) or e-mail  (arthur.goshtasby at wright.edu).
>
>
> Thank you,
> Arthur Goshtasby _______________________________________________
> 3dem mailing list
> 3dem at ncmir.ucsd.edu
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem

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