[3dem] GATAN freezing machine

[Chen Xu]

Dear Koji and list,

We happen to have both. But the Vitrobot we have is not the latest
version, Cryoplunge 3 is a beta testing version. We are actually one of
the beta testing sites for Cp3 who give feedback to Gatan for improving
purpose. 

Based on my personal experience, here are things I can say about them.

1. They both give reliable blotting and freezing.

2. Neither one does single side blotting (nicely, if possible).

3. For the easiness of unloading forceps and transferring grids to
storage box after plunging, Cp3 is significantly easier to use. 

Regards,
-- 
 Chen Xu Ph.D.
 Brandeis University
 Rosenstiel Center MS029
 415 South St., Waltham, MA 02454
 phone: +1 781 736 2469
 fax: +1 781 736 2419

* Koji Yonekura <yone at spring8.or.jp> [090204 09:33]:
> Hi All,
> 
> I wonder if anyone is using a GATAN freezing machine (Cryoplunge 3) and
> how it works.
> My collaborator (non EM expert) wants to freeze biological molecules
> routinely. I know good and bad points of Vitrobot. It would be great if
> anyone has tried both the machines.
> Thank you so much.
> 
> Best,
> Koji Yonekura
> 
> Koji Yonekura, Ph. D.
> Biostructural Mechanism Laboratory
> RIKEN SPring-8 Center
> Harima Institute
> 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan
> Tel: +81-791-58-2837 Fax: +81-791-58-1844
> E-mail: yone at spring8.or.jp
> http://www.riken.jp/biostrmech/
> 
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