[3dem] Transfer of Spider Euler angles to UCSF Chimera

Penczek, Pawel A Pawel.A.Penczek at uth.tmc.edu
Tue Dec 15 09:38:17 PST 2009


the simplest way is to notice that chimera "views" the volume along z axis.  So, instead of transfering
the angles you can rotate the volume in spider according to pjsq angles and display the rotated
version in chimera.

I hope you can figure the rest.

Pawel A. Penczek, Ph.D.
Department of Biochemistry and Molecular Biology
The University of Texas - Houston Medical School
MSB 6.220
6431 Fannin
Houston, TX 77030

phone: 713-500-5416
fax: 713-500-6297
pawel.a.penczek at uth.tmc.edu
From: 3dem-bounces at ncmir.ucsd.edu [3dem-bounces at ncmir.ucsd.edu] On Behalf Of kusser at lmb.uni-muenchen.de [kusser at lmb.uni-muenchen.de]
Sent: Tuesday, December 15, 2009 9:13 AM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] Transfer of Spider Euler angles to UCSF Chimera


I am trying to make surface representations of a Spider volume in Chimera. They should be the same view like reference projections that were used to align the data in Spider but it seems that I cannot figure out how to transfer the Euler angles from a reference angles file (coming from the Spider VO EA command and which are then used in the PJ 3Q command to create the actual reference projection images) over to Chimera to create the corresponding surface views.

Any help will be highly appreciated!


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