[3dem] Arginine and virus clump
Bill Tivol
tivol at caltech.edu
Thu May 22 11:53:25 PDT 2008
On May 21, 2008, at 8:45 PM, Eduardo Sanz-Garcia wrote:
> I was wondering if that reagent would affect negatively our cryo
> (like for example glucose).
Dear Eduardo,
What effect the arginine will have on cryo specimens depends on its
concentration. Glucose used as a cryoprotectant is in the tens of
percent concentration range, and carbohydrate-containing granules,
which bubble before the rest of a cell, are even more concentrated.
If you use ~50 mM arginine, you will be far below these
concentrations, so there should not be too significant an effect. I
have not tested this, so I'm just guessing.
Yours,
Bill Tivol, PhD
EM Scientist
Electron Cryo-Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol at caltech.edu
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