Fwd: Re: [3dem] A better way for storing freezed sample grids

[Bruno Klaholz]

Dear Paul,

we also use such workshop-made boxes, just with a round cut of the 
transparent cover rather than a rectangular cut. By the way, the hex 
bolt you mention has also the advantage of finding the center easily 
with an alan key when working under liquid nitrogen.

Cheers,

Bruno





>>Date: Tue, 26 Feb 2008 15:12:35 -0500
>>To: Eduardo Sanz-Garcia <esanzgar at chem.byu.edu>
>>From: Paul Chipman <paulrc at bilbo.bio.purdue.edu>
>>Subject: Re: [3dem] A better way for storing freezed sample grids
>>Cc:
>>Bcc:
>>X-Attachments: :Macintosh HD:336840:P1010004.JPG:
>>Hi Eduardo,
>>
>>We take the larger blue grid storage boxes and drill out circular 
>>"boxes" that fit into the same recessed areas that the Gatan 
>>Transfer Station uses.  The grids are held in place by a thin piece 
>>of plastic held in place by a hex bolt.  The bolt is stainless 
>>steel.  We've tried nylon screws but they often sheer off after 
>>repeated use of improper drying between uses.  One can store 30-40 
>>of these in a Falcon tube immersed in LN2.  We have hundreds in 
>>storage.  Other than the occasional fracturing from over 
>>tightening, they last for years.  A machine shop can produce these 
>>for a fraction of the cost of purchasing new.  The web page Norm 
>>provided looks to have similar products.  I've attached a photo but 
>>am not sure if this site permits images.
>>
>>Cheers,
>>Paul
>>
>>
>>>Currently, I am using the circular boxes that contain 4 holes to 
>>>store the already vitrified grids. I number the boxes and keep 
>>>them in a tube inside the nitrogen container.
>>>I was wondering if there is a better way for storing those vitrified grids.
>>>Thanks.
>>>_______________________________________________
>>>3dem mailing list
>>>3dem at ncmir.ucsd.edu
>>>https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
>>
>>
>>--
>>
>>Paul Chipman
>>Director, Electron Microscopy Facility
>>Project Leader, Structural Virology EM Studies
>>Dept. of Biology, Purdue University
>>Lilly Hall, Rm. B216
>>Phone: 765-494-1487
>>Fax:765-496-1189
>
>
>
>--
>Paul Chipman
>Director, Electron Microscopy Facility
>Project Leader, Structural Virology EM Studies
>Dept. of Biology, Purdue University
>Lilly Hall, Rm. B216
>Phone: 765-494-1487
>Fax:765-496-1189
>
>_______________________________________________
>3dem mailing list
>3dem at ncmir.ucsd.edu
>https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem








############################################################################

Dr. Bruno P. Klaholz
Department of Structural Biology and Genomics
Institute of Genetics and of Molecular and Cellular Biology
IGBMC - UMR 7104
1, rue Laurent Fries
BP 10142
67404 ILLKIRCH CEDEX
FRANCE


Tel. from abroad: 0033.388.65.57.55
Tel. inside France: 03.88.65.57.55

Fax from abroad: 0033.388.65.32.76
Fax inside France: 03.88.65.32.76
e-mail: klaholz at igbmc.u-strasbg.fr
websites:
http://igbmc.fr/recherche/Dep_BSG/Eq_BKlah/index_uk.html

-------------- next part --------------
An HTML attachment was scrubbed...
URL: https://mail.ncmir.ucsd.edu/mailman/private/3dem/attachments/20080227/724cf09c/attachment.html