[3dem] Zeiss SCAI/ ZI Photoscan discontinued

Terje Dokland dokland at uab.edu
Thu Nov 15 12:03:51 PST 2007


Sorry, I guess you answered my question already.
Terje

On Nov 15, 2007, at 1:53 PM, Steve Ludtke wrote:

> We originally used an in-house modified glassless holder, and in  
> fact, that's
> what we used on the 4A GroEL structure. Eventually we had too many  
> problems with
> films that weren't flat, and built some glass holders using the  
> FH869 holder as
> a base. We found that, while they were ok, there was variation in  
> quality in the
> Nikon glass holders, and one would observe focus variation across  
> the film. By
> carefully measuring the MTF across the film, and using varying  
> numbers of pieces
> of scotch tape, with epoxy once optimized, we managed to get a  
> glass holder that
> had very good performance across the film. You do lose some  
> fraction of the
> film, perhaps 10-15%, but we simply accept this limitation.
>
> ---------------------------------------------------------------------- 
> ------
> Steven Ludtke, PhD              |        Baylor College of Medicine
> sludtke at bcm.tmc.edu             |     Associate Professor & Co- 
> Director
> stevel at alumni.caltech.edu       | National Center For  
> Macromolecular Imaging
> V: (713)798-9020                |    Dept of Biochemistry and Mol.  
> Biol.
> F: (713)798-1625                |
>                                 |             Those who Do, Are
> http://ncmi.bcm.edu/~stevel     |         The converse also applies
>



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