[3DEM] Gold Labeling Workshop Announcement

hainfeld at bnl.gov hainfeld at bnl.gov
Wed Feb 28 17:10:31 PST 2007



Gold Labeling Workshop

Sept. 11-13, 2007
Sponsored by the Brookhaven National Lab STEM (scanning transmission EM) 
Facility
A Hands-on Tutorial that will cover both theory and practice.  Topics will 
include:
Gold nanoparticles:  structure, synthesis, and properties
Molecular labeling strategies (to amines, thiols, genetic tags, active 
sites, complexes, nucleic acids, peptides)
Ni-NTA-gold
Sizes of gold from 0.8 nm to 30 nm
Isolation of labeled products (various chromatographies and other methods)
Unstained, negatively stained and Cryo samples
Comparison of STEM and TEM
Silver and gold enhancement
Image processing using gold labels
Problems and how to overcome them (e.g., labeling yield/occupancy, 
non-specific binding)
Other uses of gold nanoparticles (Western blots, light microscopy, medicine)
Laboratory participation to gold label a protein for EM
What's new

Limited Enrollment (12) due to lab space:  A course fee of $650 covers 
housing, meals (dorm, cafeteria), and materials.
Proposals to attend will be refereed: submit CV and letter describing you 
and your lab's labeling interest.
Optionally describe a sample of yours that, if selected, would be labeled 
during the course.
Submit registration request by July 1 to: Dr. James Hainfeld by email: 
<mailto:hainfeld at bnl.gov>hainfeld at bnl.gov
Brookhaven National Laboratory, Biology Dept., Bldg. 463, Upton, NY  11973, USA
Tel.  631-344-3372  *  Fax. 631-344-3407


Dr. James F. Hainfeld  *  hainfeld at bnl.gov
Brookhaven National Laboratory  *  Biology Dept.  *  Bldg. 463  *  Upton, 
NY  11973   USA
Tel.  631-344-3372  *  Fax. 631-344-3407
www.biology.bnl.gov/stem/stem.html 
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