[3DEM] 626 cryoholder pin

[Bill Tivol]

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On May 1, 2006, at 11:59 PM, Robert Morrison wrote:

> Just a quick note re the construction of the holders. The end tip and 
> pin
> are machined from a single solid piece of metal in order to make them 
> as
> strong as possible (i.e. no soldered or threaded joints). 
> Unfortunately, we
> cannot choose the pin diameter. If the dewar is used to turn the 
> holder in
> the airlock there is a lot more torque available than with the standard
> holder. We have often seen broken pins where the holder was not pushed 
> fully
> in to the goniometer or when the user has turned it past the mark on 
> the
> goniometer cover when removing the holder.
>
Hi Bob,
	Thank you for your note.  I can see in the dissecting scope that the 
pin is a single piece the base of which is square.  I cannot, of 
course, see below the outside of the rod, but it does not look as 
though the process of turning down the cylindrical end of the pin from 
the square base would make the innermost part of the cylinder prone to 
the breakage that we have experienced.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol at caltech.edu
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