[3DEM] FSC Fourier Shell Correlation program available NOW!

[Marin van Heel]

The Fourier Shell Correlation (FSC) is the standard resolution
curve for three-dimensional reconstructions of single particles.  

A free download of the FSC program is now available from: 
http://www.imagescience.de/fsc <http://www.imagescience.de/fsc>.

This distribution is supported by a grant of the European Union: FP6 / 
NOE http://www.3dem-noe.org/. <http://www.3dem-noe.org/>

This version of the IMAGIC FSC program is presented with a Java 
Graphical User Interface (GUI).
Conversions from 3D data formats other than IMAGIC are possible through 
the "import" option
which uses the EM2EM universal EM converter.

 It was shown in a recent paper (van Heel M., Schatz M:* *Fourier Shell 
Correlation Threshold Criteria,* */
J. Struc. Biol./* *151 (2005) 250-262.), that all fixed value threshold 
criteria like *
"0.5" *or* "0.143"*, are *incorrect*! 

The better resolution threshold curve is the *½-bit curve* which is now 
generated by the
FSC programs. The program also plots the classical 3-sigma criterion 
indicating at
which resolution one has collected data significantly above the 
background noise level.

Moreover, the program takes into account all factors that influence the 
FSC curve
including: pointgroup symmetry of the structure and its size within the 
reconstruction volume.

Have fun!

Marin van Heel

================================================================

       Marin van Heel

       Professor of Structural Biology

       Division of Molecular Biosciences
       Faculty of Natural Sciences
       Imperial College London
       Biochemistry Building
       South Kensington Campus 
       London SW7 2AZ,        UK
 
       Tel:	   + 44 (0)20 7594 5316
       Fax:	   + 44 (0)20 7594 5317
       Priv.tel.   + 44 (0)20 8286 4248  

       e_mail: m.vanheel at ic.ac.uk
       and:    Osnat Lipson <vanheel.office at ic.ac.uk>
       web:    www.cbem.ic.ac.uk

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