[3DEM] model building for reference projections

[Sharon G. Wolf]

Adam,
HLXSIMUL, which can be found in the MRC package,
creates simulated projection images of model helices.
The MRC documentation explains how to use it.
   Good luck,
 
--Sharon Wolf
 
_____________________________
Sharon Grayer Wolf, PhD.
Electron Microscopy Unit
Weizmann Institute of Science
Rehovot 76100 Israel
email: Sharon.Wolf at weizmann.ac.il
tel:  972-8-934-4421
fax: 972-8-934-4161
_____________________________

>>> Adam Frost <adam.frost at yale.edu> 08-Oct-05 7:30:19 PM >>>

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I am a new member of the 3dem list. We are working on a large,
pseudo-helical filament imaged in ice. We are struggling to index the
helical lattice and determine the selection rule, as the FFT only has
layer
lines out to ~25 Ang from raw images. However, we think we know what
the
helical symmetry should be, both from what we know about our protein
and
from features in the FFT. 

I would like to build a 3D model of our expectations, in order to
calculate
projections and transforms, and then to test our assumptions by
comparing
the expected and observed ffts. I have no idea which software package
would
be best suited to building such a model, projections from it, or
transforms
of those projections. Any suggestions will be greatly appreciated. 

Many thanks, --Adam



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