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<DIV>Adam,</DIV>
<DIV>HLXSIMUL, which can be found in the MRC package,</DIV>
<DIV>creates simulated projection images of model helices.</DIV>
<DIV>The MRC documentation explains how to use it.</DIV>
<DIV> Good luck,</DIV>
<DIV> </DIV>
<DIV>--Sharon Wolf</DIV>
<DIV> </DIV>
<DIV>_____________________________<BR>Sharon Grayer Wolf, PhD.<BR>Electron Microscopy Unit<BR>Weizmann Institute of Science<BR>Rehovot 76100 Israel<BR>email: <A href="mailto:Sharon.Wolf@weizmann.ac.il">Sharon.Wolf@weizmann.ac.il</A><BR>tel: 972-8-934-4421<BR>fax: 972-8-934-4161<BR>_____________________________<BR><BR>>>> Adam Frost <adam.frost@yale.edu> 08-Oct-05 7:30:19 PM >>><BR></DIV>
<DIV style="COLOR: #000000">**** Messages to this list are automatically archived ***<BR>**** Please limit quoting of previous postings to the bare minimum ****<BR><BR>I am a new member of the 3dem list. We are working on a large,<BR>pseudo-helical filament imaged in ice. We are struggling to index the<BR>helical lattice and determine the selection rule, as the FFT only has layer<BR>lines out to ~25 Ang from raw images. However, we think we know what the<BR>helical symmetry should be, both from what we know about our protein and<BR>from features in the FFT. <BR><BR>I would like to build a 3D model of our expectations, in order to calculate<BR>projections and transforms, and then to test our assumptions by comparing<BR>the expected and observed ffts. I have no idea which software package would<BR>be best suited to building such a model, projections from it, or transforms<BR>of those projections. Any suggestions will be greatly appreciated. <BR><BR>Many thanks, --Adam<BR><BR><BR><BR>--<BR>For information on how to subscribe/unsubscribe/etc., send mail to<BR>3dem-request@3dem.ucsd.edu with the text "help" in the body of the<BR>message.<BR></DIV></BODY></HTML>